Journal: Pharmacological research

Article Title: CircRNA CDR1as affects functional repair after spinal cord injury and regulates fibrosis through the SMAD pathway.

doi: 10.1016/j.phrs.2024.107189

Figure Lengend Snippet: Fig. 4. CDR1as regulates NSCs differentiation and microglia polarization. NSCs differentiation was identified by immunofluorescence. GFAP staining was used to detect astrocytes and MAP2 was used to detect nerve cells (A-B). The differentiation was further identified by PCR. The characteristics of nerve cells included MAP2, Nestin and TUJ1, and the characteristics of astrocytes were GFAP (C). iNOS (D) and Arg-1 (E) were detected by immunofluorescence after the BV2 model was established in vitro. Quantitative analysis of immunofluorescence staining (F). Expression of proinflammatory cytokines iNOS, IL-6, TNF-α (G) and anti-inflammatory cytokines Arg-1, CD206 and IL-4 (H) by PCR. *P<0.05, **P<0.01, ***P<0.001.

Article Snippet: The immunofluorescence steps of NSCs and BV2 differentiation are consistent with the identification of fibroblast, and primary antibodies were: inducible nitric oxide synthase (iNOS,6022, Proteintech, Rosemont, USA), Arginase-1 (Arg-1, 16001–1-AP, Proteintech), GFAP (3670, CST), and microtubule-associated protein 2 (MAP2, 17490–1-AP, Proteintech).

Techniques: Immunofluorescence, Staining, In Vitro, Expressing

Journal: Heliyon

Article Title: Influence of green tea on alcohol aggravated neurodegeneration of cortex, cerebellum and hippocampus of STZ-induced diabetic rats

doi: 10.1016/j.heliyon.2023.e17385

Figure Lengend Snippet: Protective effect of GTE against nNOS protein expression of a. Cortex, b. Cerebellum and c. Hippocampus in alcohol administered diabetic rats ( A and 4B). Values are mean ± SEM (n = 8) in each group. A p < 0.05 is considered as statistically significant between groups and was denoted by different symbols.

Article Snippet: The complete structure of neuronal nitric oxide synthase (nNOS) was retrieved from Brookhaven Protein Data Bank (PDB Code: 6NG1 ) [ ], in which, the ligand molecule (KLY), water molecules were removed and heme group (HEM) and substrate (H4B) were keep in the active site.

Techniques: Expressing

Journal: Heliyon

Article Title: Influence of green tea on alcohol aggravated neurodegeneration of cortex, cerebellum and hippocampus of STZ-induced diabetic rats

doi: 10.1016/j.heliyon.2023.e17385

Figure Lengend Snippet: Optimized structures of (a) Epigallocatechin gallate (b) Glibenclamide and (c) Rosiglitazone molecules using B3LYP/6-311G** method ( A). The intermolecular interactions of (a) Epigallocatechin gallate in the active site of nNOS (Neuronal Nitric Oxide Synthase) enzyme (b) the 2D representation of their intermolecular interactions and (c) surface view ( B). The intermolecular interactions of (a) Glibenclamide in the active site of nNOS (Neuronal Nitric Oxide Synthase) enzyme (b) the 2D representation of their intermolecular interactions and (c) surface view ( C). The intermolecular interactions of (a) Rosiglitazone in the active site of nNOS (Neuronal Nitric Oxide Synthase) enzyme (b) the 2D representation of their intermolecular interactions and (c) surface view ( D).

Article Snippet: The complete structure of neuronal nitric oxide synthase (nNOS) was retrieved from Brookhaven Protein Data Bank (PDB Code: 6NG1 ) [ ], in which, the ligand molecule (KLY), water molecules were removed and heme group (HEM) and substrate (H4B) were keep in the active site.

Techniques:

Journal: Heliyon

Article Title: Influence of green tea on alcohol aggravated neurodegeneration of cortex, cerebellum and hippocampus of STZ-induced diabetic rats

doi: 10.1016/j.heliyon.2023.e17385

Figure Lengend Snippet: Binding free energy values of Neuronal Nitric Oxide Synthase (nNOS) inhibitors.

Article Snippet: The complete structure of neuronal nitric oxide synthase (nNOS) was retrieved from Brookhaven Protein Data Bank (PDB Code: 6NG1 ) [ ], in which, the ligand molecule (KLY), water molecules were removed and heme group (HEM) and substrate (H4B) were keep in the active site.

Techniques: Binding Assay